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Overview of in vitro methods used to assess e-cigarettes based on “Toxicity Testing in 21st Century” principles

Due to the regulatory landscape and dynamic nature of innovation within the e-cigarette category, new assays are required to quickly determine any subtle biological responses these products may elicit for product stewardship
purposes. In these studies, e-liquids and/or aerosol were assessed using a number of biological endpoints
to understand cytotoxic, inflammatory and oxidative responses.

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