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Overview of in vitro methods used to assess e-cigarettes based on “Toxicity Testing in 21st Century” principles

Neat e-liquids and aerosol did not generally elicit a toxicological response in a range of in vitro assays at physiologically relevant concentrations (except dermal irritation), our findings are consistent with other researchers. We believe these in vitro assays provide useful tools as part of a wider safety assessmentfor e-cigarettes.

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