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The use of human 3D reconstructed bronchial tissue to study the effects of cigarette smoke and e-cigarette aerosol on a wide range of cellular endpoints

In 2015, Public Health England characterised e-cigarettes as being around 95% less harmful than smoking. In 2016, the UK Royal College of Physicians concluded that the long-term health risks associated with e-cigarettes are unlikely to exceed 5% of those associated with smoked tobacco products and maybe substantially less. However, some recent data has reported that e-cigarette aerosol can potentially produce reactive oxygen species which may give rise to inflammation, DNA damage and reduced cell viability. To investigate these claims, we studied the effect of two different e-liquid aerosols on EpiAirway 3D tissue and a variety of biological endpoints.

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